rabbit anti gbp5  (Boster Bio)

Journal: Communications biology

Article Title: STAT1 mediates the pro-inflammatory role of GBP5 in colitis.

doi: 10.1038/s42003-025-07843-0

Figure Lengend Snippet: Fig. 3 | The effect of GBP5 knockout on the global mRNA expression mirrors that of STAT1 activa- tion in THP-1 cells. a Plots are the mRNA expres- sion data of STAT1 and STAT1 target genes from RNA sequencing-based transcriptome analysis. Wildtype and GBP5-/- THP-1 cells were treated with IFNγ (25 ng/ml) plus LPS (500 ng/ml) or mock- treated for 16 h. The list of the STAT1 target genes was from TRRUST version 2, with a few others (CCND1, MYC, and CDC25A) added according to the literature. Genes known to be stimulated and repressed by STAT1 are indicated by red and green, respectively. n = 3 biologically independent samples for each group. b Gene set enrichment analysis (GSEA) of the entire transcriptome data of GBP5-/-

Article Snippet: We used anti-mouse GBP5 (1:200, Abcam, Cat#ab313390), anti-mouse F4/80 (1:200, Abcam, Cat#ab6640), anti-mouse CD127/IL-7Rα (1:200, CST, Cat#47903), antimouse CD45R/B220 (1:200, Biolegend, Cat#103235), anti-human GBP5 (1:200, CST, Cat#67798S) and anti-mouse/human STAT1 (1:200, Proteintech, Cat#66545-1-Ig) as primary antibodies, Alexa Fluor 488- conjugated goat anti-rabbit (Invitrogen, Cat#A32731), Alexa Fluor 594- conjugated goat anti-rabbit (Invitrogen, Cat#A11012), Alexa Fluor 594- conjugated goat anti-mouse (Invitrogen, Cat#A11005), DyLight 594 AffiniPure goat anti-rat (EarthOx, Cat#E032440-01) and DyLight 488 AffiniPure goat anti-rat (EarthOx, Cat#E032240-01) as secondary antibodies.

Techniques: Knock-Out, Expressing, RNA Sequencing

Journal: Communications biology

Article Title: STAT1 mediates the pro-inflammatory role of GBP5 in colitis.

doi: 10.1038/s42003-025-07843-0

Figure Lengend Snippet: Fig. 6 | Binding of GBP5 to STAT1 promotes the nuclear translocation of STAT1. a Immunofluorescence staining of THP-1 cells untreated or treated with IFNγ/LPS using antibodies against GBP5 and STAT1. Scale bar: 5 μm. b Subcellular fractionation analysis of GBP5 and STAT1. WT and GBP5-/- THP-1 cells were untreated or treated with IFNγ/LPS for 16 h, harvested to prepare nuclear and cytoplasmic fractions, and analyzed by Western blot with antibodies against GBP5, STAT1, and p-STAT1 (S727). GAPDH and Lamin B were used as internal references. The bar plot on the right was the quantification of three independent experiments. n = 3 biologically inde- pendent experiments for each group. Data are mean ± SEM, paired student t-test. c GBP5-STAT1 proximity ligation assay with THP-1 cells untreated or treated with IFNγ/LPS. Each red spot represents an interaction. Scale bar: 2 μm. Nuclei were stained with DAPI (blue).

Article Snippet: We used anti-mouse GBP5 (1:200, Abcam, Cat#ab313390), anti-mouse F4/80 (1:200, Abcam, Cat#ab6640), anti-mouse CD127/IL-7Rα (1:200, CST, Cat#47903), antimouse CD45R/B220 (1:200, Biolegend, Cat#103235), anti-human GBP5 (1:200, CST, Cat#67798S) and anti-mouse/human STAT1 (1:200, Proteintech, Cat#66545-1-Ig) as primary antibodies, Alexa Fluor 488- conjugated goat anti-rabbit (Invitrogen, Cat#A32731), Alexa Fluor 594- conjugated goat anti-rabbit (Invitrogen, Cat#A11012), Alexa Fluor 594- conjugated goat anti-mouse (Invitrogen, Cat#A11005), DyLight 594 AffiniPure goat anti-rat (EarthOx, Cat#E032440-01) and DyLight 488 AffiniPure goat anti-rat (EarthOx, Cat#E032240-01) as secondary antibodies.

Techniques: Binding Assay, Translocation Assay, Staining, Fractionation, Western Blot, Proximity Ligation Assay